clarity max western ecl substrate (Bio-Rad)
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clarity max western ecl substrate
Clarity Max Western Ecl Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 2688 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clarity+max+western+ecl+substrate/Clarity+Max+Western+ECL+Substrate/pmc13651822-284-12-17
Average 97 stars, based on 2688 article reviews
Clarity Max Western Ecl Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 2688 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clarity+max+western+ecl+substrate/Clarity+Max+Western+ECL+Substrate/pmc13651822-284-12-17
Average 97 stars, based on 2688 article reviews
clarity max western ecl substrate - by Bioz Stars,
2026-10
97/100 stars
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Western Blot:Article Title: Engineering small peptide secretion in Komagataella phaffii Article Snippet: Membranes were blocked in TBST with 5% BSA for 3 h at room temperature with gentle agitation, then incubated with ANTI-FLAG M2 Peroxidase (HRP) antibody (Sigma-Aldrich; 1:1500 in TBST with 2.5% BSA) overnight at 4 °C. .. After three washes with TBST (5 min each), signal was detected using the Article Title: Stroma-oriented tumor microenvironment facilitates intraosseous ameloblastoma tumor growth. Article Snippet: Membranes were incubated overnight at 4◦C with primary antibodies, including LGR5 (Invitrogen, #MA5-25644), non-phospho (Active) β-catenin (Cell Signaling Technology, #8814), fibronectin (Sigma, #F3648), ALDH1 (BD biosciences, #611194), YAP (Cell Signaling Technology, #14074), E-Cadherin (Cell Signaling Technology, #3195), CD63 (Invitrogen, #10628D), CD9 (Invitrogen, #10626D), CD81 (Invitrogen, #10630D), β-actin (Santa Cruz, #sc-47778), followed by horseradish peroxidase-conjugated secondary antibodies for 1 hour. .. Protein bands were visualized using Article Title: Targeting O 6 -methylguanine-DNA methyltransferase deficiency in preclinical models of extracranial human cancers with a tumor-selective DNA modifying agent Article Snippet: Membranes were blocked for 5–10 min in EveryBlot Blocking Buffer (Bio-Rad) and incubated with primary and secondary antibodies ( Supplemental Table S1 ). .. Chemiluminescent detection was performed on a ChemiDoc XRS+ Molecular Imager (Bio-Rad) or a ChemiDoc Go Imaging System (Bio-Rad) using Clarity Western ECL Substrate (Bio-Rad) or Article Title: A4gnt-Deficient Mice Show Enhanced IL-11 Receptor α-Glycoprotein 130-Janus Kinase 2-STAT3 Signaling in Early Stages of Gastric Tumorigenesis: Comparative Analysis with A4gnt and Il11 Doubly Deficient Mice. Article Snippet: From the Department of Molecular Pathology, Shinshu University School of Medicine, Matsumoto; the Laboratory of Biomedical Science and the Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, and the Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo; the Shinshu University School of Medicine, Matsumoto; the Department of Biotechnology, Institute for Biomedical Sciences, Research Cluster for Social Implementation, Shinshu University, Matsumoto; the Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto; and the Division of Integrated Research, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Japan Article Title: Phylogenomic Diversity, Serotype Heterogeneity and In Vivo Virulence of Flavobacterium psychrophilum Isolates From Rainbow Trout Farms in Türkiye. Article Snippet: Following the washing steps, membranes were incubated with HRP- conjugated goat anti- rabbit IgG secondary antibody (Santa Cruz Biotechnology, Dallas, TX, USA) at a dilution of 1:200. .. Immunoreactive bands were detected using Article Title: Inducible dCas12a system expands CRISPRi/a capabilities for functional genomics in malaria parasite Plasmodium falciparum Article Snippet: To assess the expression of SET7 in parasites before and after DiCre induction, Western blots were performed with rabbit anti-SET7 (1:50) and rabbit anti-PfAldolase antibodies as primary antibodies, followed by HRP-conjugated goat anti-rabbit IgG (Sigma) as secondary antibodies ( ). .. Proteins were separated by SDS-PAGE, transferred to membranes and detected using the Article Title: NanoLuciferase Reporter Assays to Investigate Protein Dynamics and Circadian Clock Regulation in the Diatom Model Species Phaeodactylum tricornutum. Article Snippet: For detection of the RITMO1 tagged protein, membranes were blocked 1h in 1x PBS, 5% milk and 0.1% Tween 20, and then incubated overnight at 4°C with a 1/5000 dilution of purified mouse monoclonal antibody HA.11 from Covance (cat no.MMS-101R) in 1x PBS, 5% milk and 0.1% Tween 20. .. HRP-conjugated anti-mouse secondary antibodies (Promega, W402B) at a 1/10 000 dilution were subsequently incubated for 1 h before visualization using Imaging:Article Title: Engineering small peptide secretion in Komagataella phaffii Article Snippet: Membranes were blocked in TBST with 5% BSA for 3 h at room temperature with gentle agitation, then incubated with ANTI-FLAG M2 Peroxidase (HRP) antibody (Sigma-Aldrich; 1:1500 in TBST with 2.5% BSA) overnight at 4 °C. .. 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Immunoreactive bands were detected using Article Title: Inducible dCas12a system expands CRISPRi/a capabilities for functional genomics in malaria parasite Plasmodium falciparum Article Snippet: To assess the expression of SET7 in parasites before and after DiCre induction, Western blots were performed with rabbit anti-SET7 (1:50) and rabbit anti-PfAldolase antibodies as primary antibodies, followed by HRP-conjugated goat anti-rabbit IgG (Sigma) as secondary antibodies ( ). .. Proteins were separated by SDS-PAGE, transferred to membranes and detected using the Article Title: Somatic DDX41 mutations confer neomorphic splicing activity in the pathogenesis of myelodysplastic neoplasms Article Snippet: .. Blots were imaged using Clarity (BioRad, 1705060) or Pathology:Article Title: A4gnt-Deficient Mice Show Enhanced IL-11 Receptor α-Glycoprotein 130-Janus Kinase 2-STAT3 Signaling in Early Stages of Gastric Tumorigenesis: Comparative Analysis with A4gnt and Il11 Doubly Deficient Mice. Article Snippet: From the Department of Molecular Pathology, Shinshu University School of Medicine, Matsumoto; the Laboratory of Biomedical Science and the Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, and the Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo; the Shinshu University School of Medicine, Matsumoto; the Department of Biotechnology, Institute for Biomedical Sciences, Research Cluster for Social Implementation, Shinshu University, Matsumoto; the Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto; and the Division of Integrated Research, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Japan Incubation:Article Title: NanoLuciferase Reporter Assays to Investigate Protein Dynamics and Circadian Clock Regulation in the Diatom Model Species Phaeodactylum tricornutum. Article Snippet: For detection of the RITMO1 tagged protein, membranes were blocked 1h in 1x PBS, 5% milk and 0.1% Tween 20, and then incubated overnight at 4°C with a 1/5000 dilution of purified mouse monoclonal antibody HA.11 from Covance (cat no.MMS-101R) in 1x PBS, 5% milk and 0.1% Tween 20. .. HRP-conjugated anti-mouse secondary antibodies (Promega, W402B) at a 1/10 000 dilution were subsequently incubated for 1 h before visualization using |